Wednesday, November 02, 2005

Western Blot Test

The Western Blot Test showed David does produce Wiskott-Aldrich Protein in his T-cells. The B-cells did not grow properly for the test so the Dr. will try to grow another line of B-cells from the few million cells they have left of David's frozen blood samples.

The evidence of WAS protein was good news. When the flow cytometry test was done, the histograms showed no WAS protein in the T-cells and very little in the B-cells. I'm not sure if there is a clear explanation between the discrepancies of the flow cytometry and the Western Blot tests. The Dr. said flow cytometry results are obtained quicker than Western Blot. The Western Blot is time consuming, requiring the cells to be separated and many more procedures.

The Dr. confirmed by visual inspection of the Western Blot test, that David produces a similar amount of protein compared with other X-linked Thrombocytopenia patients. This was also good news.

The puzzle is not completely finished yet. David has a second opinion scheduled with a Dr. at UCSF on Nov 17. We hope she will give us better advice or recommendations for David's future health care.

Meanwhile, I am still, yes, still learning about Wiskott-Aldrich. I cannot believe how complicated this disorder is.

Pleas pray that within the next 1-2 months we will be able to put the last pieces to this complicated puzzle together and make a wise decision about whether to proceed with the BMT or not.

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